Recombinant Human BMP-4 | Ajinomoto Group

Product Information
BMP-4

10 μg (0.1 mg/ml, 100 μl)
SP-BMP4-R-010UG

50 μg (0.1 mg/ml, 500 μl)
SP-BMP4-R-050UG

1 mg (0.1 mg/ml, 10 ml)
SP-BMP4-R-001MG

GMP compliant product is in preparation. Contact us for further information.

Significant cost reduction of cell
manufacturing processes

Seamless transition from basic research
to clinical applications

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NAME	Recombinant Human BMP-4
SOURCE	E. coli
FORMULATION	20 mM Gly-HCl Buffer pH 2.3
Quality	Animal origin free
AA Sequence:	MKKNKNCRRH SLYVDFSDVG WNDWIVAPPG YQAFYCHGDC PFPLADHLNS TNHAIVQTLV NSVNSSIPKA CCVPTELSAI SMLYLDEYDK VVLKNYQEMV VEGCGCR
Purity:	≥ 95% by SDS-PAGE
Endotoxin	<0.1EU/ug
Description:	BMP-4 (Bone morphogenetic protein-4) is a protein that plays an important role in metabolism of bone and cartilage. And many organ cells are influenced by BMP-4 signal. In addition, BMP-4 has been shown to be involved in the progression of diseases, such as bone diseases. Recombinant Human BMP-4 is a 24.1kDa, disulfide-linked homodimeric protein consisting of two 107 amino acid polypeptide chains. It corresponds to amino acids 303-408 of the full-length BMP-4 precursor with an N-terminal Met.

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	AJINOMOTO	Company A	Company B
Form	Frozen (0.1 mg/ml) in 20 mM Gly-HCI Buffer pH 2.3	Lyophilized	Lyophilized
Sterilization	0.22 μm filtration	0.2 μm filtration	0.2 μm filtration
Host	E. coli	E. coli	E. coli
Primary structure	10 AA truncated	10 AA truncated	10 AA truncated
Protein Content	0.100-0.140 mg/ml (HPLC)	ー	ー (UV and/or SDS-PAGE)
Purity	≧95% (SDS-PAGE)	>95% (SDS-PAGE)	>98% (SDS-PAGE)
Endotoxin	<0.1 EU/μg (LAL method)	<0.10 EU/μg (LAL method)	<1 EU/μg
Biological activity	EC₅₀: 30-120 ng/ml^*1 alkaline phosphatase production by ATDC-5 Cells	EC₅₀: 6-48 ng/ml^*2 alkaline phosphatase production by ATDC-5 Cells	EC₅₀: 5-10 ng/ml^*2 alkaline phosphatase production by ATDC-5 Cells
N-terminal	Met-Lys-Lys	Lys	Lys-Lys-Lys

As of 2023 Dec.

ALP assay (In-house purified sample)

AJINOMOTO’s BMP-4 demonstrates equal to biological
activity compared to tested alternatives!

	AJINOMOTO	Company A	Company B
Storage time (temperature)	4 years from manufacturing date (-80˚C)	1 week from reconstitution date (2˚C to 8˚C)	3 months from reconstitution date (under -70˚C)
Freeze-thaw cycles	Within 3 times^*	ー	ー