StemFit For Differentiation
StemFit For Diff. combined with StemFit medium for the hPSCs expansion can enable clinical applications of hPSC-derived cells/tissues by providing the CD-AOF culture systems for both hPSCs expansion and differentiation.
StemFit For Diff. is provided as a 5×concentrate and is intended to be used with basal medium (DMEM/F12、RPMI1640、DMEM etc.) and a variety of different induction factors or cytokines.
— Materials Provided —
||Store at below -20 °C
— Prepare Differentiation Basic Medium —
StemFit For Diff. is provided frozen and should be stored at below -20 °C until use. Use sterile techniques to prepare differentiation medium as follow.
1) Before use, thaw the frozen StemFit For Diff. with occasionally mixing at room temperature (15-25 °C) or overnight at 4°C.
CAUTION: Do not thaw StemFit for Diff. at 37 °C, as it accelerates the degradation of medium
2) Add 100 mL of StemFit For Diff. (5X) to 400 mL basal medium (DMEM/F12、RPMI1640、DMEM etc.) and mix thoroughly to make differentiation basic medium. If precipitations are observed, keep the bottle at room temperature and dissolve them.
Optional: StemFit For Diff. and differentiation basic medium may be aseptically aliquoted and stored at below -20 °C. Once thawed, they may be stored at 2-8 °C for up to 2 weeks (Do not re-freeze.). We recommend storing the medium protected from light.
3) To differentiate each lineage, induction factors or cytokines can be added as specified by differentiation protocols.
4) Before use, warm aliquots to room temperature and use immediately.