Feeder free medium for ES/iPS cells StemFit

Please contact us

stemfit@ajinomoto.com

stemfit@ajinomoto.com
iPSC/ESC medium Feeder free medium for ESC/iPSC Cells iPSC/ESC medium Feeder free medium for ESC/iPSC Cells
PAGE TOP

Product

StemFit Basic02 StemFit Basic02

StemFit Basic02 is a xeno-free,
defined medium for human
pluripotent stem cell culture.

StemFit Basic03 StemFit Basic03

StemFit Basic03 is an animal-origin
free, defined medium for human
pluripotent stem cell culture.

StemFit Basic03 GMP StemFit Basic03 GMP

StemFit Basic03 GMP is an
animal-origin free, defined medium for
human pluripotent stem cell culture.

StemFit Basic04 StemFit Basic04

StemFit Basic04 is an animal-origin
free, defined medium with one bottle
formula for human pluripotent
stem cell culture.

StemFit Basic04 StemFit Basic04
Complete Type

StemFit Basic04 Complete Type
(Basic04CT) is an animal-origin free,
defined medium for human
pluripotent stem cell culture.
*This medium contains bFGF.

StemFit AK02N/03N StemFit AK02N/AK03N

StemFit AK02N and StemFit AK03N
are corresponding to Basic02 and
Basic03 medium respectively but it
contains bFGF. These products are
commercially available only in Japan.

Features

Product comparison table

Product comparison table

Significant cost reduction

Significant cost reduction Significant cost reduction

StemFit media enables flexible weekend-free feeding which drastically reduces the culture cost.

Superior growth performance on any matrices

Superior growth performance on any matrices

Human 201B7 iPSCs grown on MEFs (feeder-dependent) were transitioned to feeder-free conditions with StemFit Basic02 or commercially available medium A on respective ECMs (1000 cells/cm2), and cultured for one week.

Superior colony-forming efficiency from a single cell clone

Superior colony-forming efficiency from a single cell clone Superior colony-forming efficiency from a single cell clone

Human iPSCs were adapted to StemFit Basic02, or commercially available medium A or B on Matrigel® for more than 3 passages. Then, cells were serially diluted and seeded with each medium on Matrigel®-coated 96-well plates at 1 cell/well or 10 cells/well. The number of seeded cells was counted after 3 hours, and colonies were counted at day 7.

Highly stable and reproducible single-cell
and feeder-free culture system

Highly stable and reproducible single-cell and feeder-free culture system Highly stable and reproducible single-cell and feeder-free culture system

Data from Cell and Gene Therapy Catapult in UK

Superior performance of StemFit AK03N for the culture of induced pluripotent stem cells
–Cell and Gene Therapy Catapult conducted the comparative cultivation testing in different culture systems–

Data from Cell and Gene Therapy Catapult in UK

The figure shows the average level of population doublings per day (PDL/day(d-1)

PDF Download
PAGE TOP
Differentiation medium MSC medium Growth Factors

For further information, please contact here.

stemfit@ajinomoto.com

AJINOMOTO CO., INC. AminoScience Division
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan
page top