StemFit Basic02 is a xeno-free, defined medium for human pluripotent stem cell culture.
StemFit Basic03 is an animal-origin free, defined medium for human pluripotent stem cell culture.
StemFit Basic04 is an animal-origin free, defined medium with one bottle formula for human pluripotent stem cell culture.
Comparison table for StemFit Basic02 and 03 and 04
|StemFit Basic02||StemFit Basic03||StemFit Basic04|
|cGMP||ー||in preparation||in preparation|
|Number of Bottle||2||2||1|
Less frequent medium changing / Lower medium volume (6-well plate)
Superior growth performance on any matrices
Human 201B7 iPSCs grown on MEFs (feeder-dependent) were transitioned to feeder-free conditions with StemFit Basic02 or commercially available medium A on respective ECMs (1000 cells/cm2), and cultured for one week.
Superior colony-forming efficiency from a single cell clone
Human iPSCs were adapted to StemFit Basic02, or commercially available medium A or B on Matrigel® for more than 3 passages. Then, cells were serially diluted and seeded with each medium on Matrigel®-coated 96-well plates at 1 cell/well or 10 cells/well. The number of seeded cells was counted after 3 hours, and colonies were counted at day 7.
Highly stable and reproducible single-cell and feeder-free culture system
Total fold expansion
Data from Cell and Gene Therapy Catapult in UK
Superior performance of StemFitAK03N for the culture of induced pluripotent stem cells
–Cell and Gene Therapy Catapult conducted the comparative cultivation testing in different culture systems–
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