StemFit™ Basic04 CT (GMP)

StemFit™ Basic04 Complete Type (GMP)

StemFit™ Basic04 Complete Type (Basic04CT) is an animal origin-free, defined medium for human pluripotent stem cell culture. This medium includes Basic Fibroblast Growth Factor (bFGF) and can be used for maintaining human ES and iPS cells under feeder-free culture conditions. It is for both research and further manufacturing use.

The QMS (Quality Management System) follows the Ajinomoto System of Quality Assurance (ASQUA). Manufacturing and product release testing of this product are managed in accordance with the GMP standards Ajinomoto formulated for culture media for regenerative medicine. The GMP standards are prepared with reference to ICHQ7 and “PMDA guidance for Aseptic Manufacturing Processes of Regenerative Medicine Products.

Click here to learn more about GMP grade.

Key Benefits

1

Seamless Support from iPSC Derivation to Expansion

2

Simpler Workflow

3

Cost Efficiency

4

One-Step Transition from Existing Media to Basic04CT

Seamless Support from iPSC Derivation to Expansion

1. Reliable Cell Establishment and Differentiation Performance

Reliable Cell Establishment and Differentiation Performance

1. (A) TRA-1-60-positive iPSC colonies were detected by immunostaining on day 14 after synthetic mRNA-based reprogramming of human PBMCs cultured in StemFit™ Basic04CT.
2. (B) G-band karyotyping was performed on iPSC clones established and maintained in StemFit™ Basic04CT, confirming normal karyotype.
3. (C) Pluripotency and trilineage differentiation potential were assessed using the TaqMan^®-based assays. RNA was extracted from both undifferentiated (Pluri QC) and spontaneously differentiated iPSCs (Diff QC) cultured in StemFit™ Basic04CT. The heatmap shows QC scores for self-renewal and germ layer–specific markers relative to a validated reference set.

*Data generated through a collaborative study with Masato Nakagawa, Ph.D. (iPS Cell Core Facility, WPI-PRIMe, The University of Osaka).

2. Cloning Performance & Karyotype Stability

(A) Single-cell Sorting Recovery

Single iPSCs were sorted into 384-well plates and cultured in Major RUO medium A + CloneR2 or Basic04CT + CloneR2.

Single-cell Sorting Recovery

(B) Frequency of Clones with Normal Karyotype

All clones expanded in Basic04CT exhibited a normal karyotype (10/10).

Frequency of Clones with Normal Karyotype

3. High Single-Cell Expansion

Achieves expansion from 10⁴ to 10⁹ cells within 3 weeks, delivering over 10⁵-fold cumulative expansion of both blood-derived (1210B2) and fibroblast-derived (201B7) iPSCs.

High Single-Cell Expansion

4. Consistent across Substrates

Blood-derived hiPSCs (1210B2) cultured in Basic04CT showed comparable fold expansion across various substrates.

Consistent across Substrates

Simpler Workflow

1. One Bottle

Eliminates supplement mixing and reduces contamination risk.

One Bottle

2. Weekend-free feeding

Skip weekend feeds for flexible scheduling and operator-friendly workflow.

Weekend-free feeding

Cost Efficiency

Lowest Weekly Cost vs. Major AOF & RUO Media

Cost Efficiency

The graph shows the estimated weekly medium cost per well (1/6-well scale) calculated from 2025 U.S. catalog list prices and each manufacturer's recommended feeding/passaging schedule.

Basic04CT achieves the lowest weekly culture cost among the major AOF and RUO medium.

One-Step Transition from Existing Media to Basic04CT

Consistent Cumulative Expansion through Medium Transition (3 passages)

Seamless switch / Comparable expansion / Workflow continuity

— Material Provided —

Volume	Storage
500 ml	Store below -20 °C

— Media Preparation —

StemFit™ Basic04 Complete Type is provided as a frozen medium and should be stored below -20 °C until use. Use sterile techniques when using this medium.

1) Before use, thaw the frozen Basic04CT with occasionally mixing at room temperature (15-25 °C).
CAUTION : Do not thaw StemFit™ Basic04 Complete Type at 37 °C, as it accelerates the degradation of the medium.
If precipitation are observed, keep the bottle at room temperature and dissolve them.1

2) After thawing, StemFit™ Basic04 Complete Type may be aseptically aliquoted and stored below -20 °C. Thawed StemFit™ Basic04 Complete Type may be stored at 2-8 °C for up to two weeks.2
We recommend storing the medium protected from light.3

3) Before use, warm aliquots at room temperature and use immediately.

Key points for successful culture