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StemFit Puroteins Recombinant Proteins Animal-origin free proteins StemFit Puroteins Recombinant Proteins Animal-origin free proteins

Product Information
KGF (FGF-7)

KGF
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— Product Size —

Non GMP

10 μg (0.1 mg/ml, 100 μl)
SP-KGF-R-010UG

50 μg (0.1 mg/ml, 500 μl)
SP-KGF-R-050UG

1 mg (0.1 mg/ml, 10 ml)
SP-KGF-R-001MG

Benefits

Significant cost reduction of cell
manufacturing processes

High lot-to-lot consistency

Seamless transition from basic research
to clinical applications

Simplified protocols without
reconstitution

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— Product Details —

NAME Recombinant Human KGF (FGF-7)
SOURCE Corynebacterium glutamicum*
FORMULATION Frozen in DPBS(-) containing 1.5mM DTT and 100mM Na2SO4
Quality Animal origin free
AA Sequence: CNDMTPEQMA TNVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK
RGKVKGTQEM KNNYNIMEIR TVAVGIVAIK GVESEFYLAM NKEGKLYAKK
ECNEDCNFKE LILENHYNTY ASAKWTHNGG EMFVALNQKG IPVRGKKTKK
EQKTAHFLPM AIT
Purity: ≥ 95% by SDS-PAGE
Endotoxin <0.1EU/ug
Description: Keratinocyte Growth Factor (KGF) is one of 23 known members of the FGF family,
and it is known as FGF-7. Proteins of this family play a central role during prenatal development,
postnatal growth, and the regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. KGF is a mitogen factor specific for epithelial cells and keratinocytes. Recombinant Human KGF is an 18.9 kDa protein consisting of 163 amino acid residues.

*Corynebacterium glutamicum

  • • Discovered in 1957 as a glutamate producer
  • • Fast growing soil bacterium
  • • Gram-positive
  • • Non-sporulating
  • • Non-pathogenic
  • • Non-endotoxic
  • • FDA approved
*Corynebacterium glutamicum

< Features >

  • Secretion System
  • No Endotoxin
  • Less Derivatives (impurities)
  • Experience: Food Amino Acid Production

Ideal platform for low-cost, high-grade proteins production with high lot-to-lot consistency.

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— Product Comparison Table —

AJINOMOTO Company A Company B
Host cell C. glutamicum E. coli E. coli
Endotoxin <0.1EU/μg
(LAL)
<1EU/μg
(LAL)
<0.10EU/μg
(LAL)
Purity 95%
(SDS-PAGE)
≧95%
(SDS-PAGE、HPLC)
>97%
(SDS-PAGE)
Biological
activity
EC50, 0.1-30 ng/mL*1 ED50, ≦10 ng/ml*2 ED50, 6-60 ng/ml*3
Form Frozen
in DPBS(-) containing
1.5 mM DTT, 100 mM Na2SO4
Lyophilized Lyophilized
N-terminal Cys-Asn Met-Cys-Asn Met-Cys-Asn
Expiration date 2 years from
manufacturing date
At least 4 years from
manufacturing date
12 months from
date of receipt
  • *1 ERK1/2 phosphorylation assay using MCF-7 cells
  • *2 Dose-dependent ability to reduce tetrazolium salt, WST-8,
    by dehydrogenase activities of BaF3 cells expressing FGF receptors
  • *3 Cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells
〈 Comparison of biological activity with other companies’ products 〉

Phosphorylation of ERK1/2

〈 Comparison of biological activity with other companies’ products 〉

AJINOMOTO’s KGF demonstrates equal biological
activity compared to tested alternatives!

〈 Stability information after reconstitution 〉
AJINOMOTO Company A Company B
-80°C 2 years
from manufacturing date
3 months from
reconstitution date
3 months from
reconstitution date
Freeze-thaw
cycles
Within 3 times
Download

・Product Information
KGF [1mg](PDF [277KB]) KGF [10μg](PDF [282KB]) KGF [50μg](PDF [280KB])

・SDS (PDF [128KB] / En) (PDF [143KB] / JP)

iPSC/ESC medium Differentiation medium MSC medium

For more information, please contact us.

Contact us
AJINOMOTO CO., INC. AminoScience Division
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan

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