Feeder free medium for ES/iPS cells StemFit

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MSC medium Chemically-defined & Animal-origin free medium MSC medium Chemically-defined & Animal-origin free medium

Product

StemFit For Mesenchymal Stem Cell

StemFit For Mesenchymal Stem Cell

StemFit For Mesenchymal Stem Cell (StemFit For MSC) is
a chemically defined medium
for mesenchymal stem cell (MSC) culture.

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Product Information
StemFit For MSC

StemFit For Mesenchymal Stem Cell (StemFit For MSC) is a chemically defined medium for mesenchymal stem cell (MSC) culture. This medium does not contain any undefined components such as serum or human platelet lysate, and it enables the maintenance of human bone marrow-derived MSC (BM-MSC), umbilical cord-derived MSC (UC-MSC) and adipose-derived stem cells (ADSC), under chemically defined conditions.

— Materials Provided —

Volume Storage
500ml Store at -20 °C

— Media Preparation —

Use sterile techniques to prepare StemFit For MSC medium.

1) Thaw StemFit medium at room temperature (15-25 °C) or at 2-8°C overnight. Mix thoroughly.
CAUTION: Do not thaw StemFit For MSC at 37 °C, as it accelerates medium degradation
> Thawed StemFit For MSC medium may be stored at 2-8 °C for up to a month. Protect from light.
> Optionally, the medium can be stored as aliquots at -20°C until the expiration date. Do not re-freeze thawed aliquots.

2) Warm medium to room temperature and use immediately.

Key points for successful culture

not 37°C

Do not thaw the media at 37°C because it accelerates degradation of media.

iMatrix-511

Add iMatrix-511 or use matrix coated plate.

Remove

Remove dissociation reagent completely after centrifugation.

not over confluent

Avoid over confluent culture because that makes it difficult to detach and collect cells.

— Precaution and Disclaimer —

StemFit For MSC is designed in accordance with the BSE/TSE guideline (EMEA/410/01 Rev.3). Any animal- and human-derived components are properly inactivated and the risk associated with the animal-derived ingredient is negligible.

We define “Chemically Defined Medium” as
1. A medium in which all of the components and concentrations are known.
2. A medium which does not contain serum, lysates, or other ingredients with unknown composition

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Features

High consistency

Free of undefined components (Serum, Human platelet lysate). Chemically defined composition minimizes lot-to-lot variation and enables consistent cell expansion.

High consistency

High Safety

Minimized risk of hazardous animal- and human-derived components. StemFit For MSC is designed in accordance with the BSE/TSE guideline (EMEA/410/01 Rev.3). Any animal- and human-derived components are properly inactivated and the risk associated with the animal-derived ingredient is negligible.

High Safety

High performance

StemFit For MSC enables superior cell expansion to serum containing media. This medium can maintain MSCs with high level of maker expression and differentiation potential.

〈 Expansion 〉
〈 MSC marker expression 〉
〈 Differentiation potential 〉
Download

・Product Information & Protocol (PDF [582KB])


・SDS (PDF [139KB] )

・Brochure (PDF [1.1MB] )

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StemFit For MSC
FAQ

  • Which type of MSCs can I expand with StemFit For MSC medium? StemFit for MSC medium is available for bone marrow-derived MSC, umbilical cord-derived MSC and adipose-derived stem cell expansion.  
  • Can I use StemFit For MSC medium for isolation of MSC? We confirmed that StemFit For MSC can be used for isolation of MSC from bone marrow and adipose tissue. Please contact us directly for detailed protocol of isolation.
  • Is any scaffold protein required? Yes, scaffold protein is required.  It is necessary to add iMatrix-511 or use ECM-coated plate such as vitronectin or fibronectin.  Please refer to the protocol for more detailed information.
  • How should I switch medium to StemFit For MSC medium? We recommend to thaw your cell stock with StemFit For MSC medium according to the protocol.
  • Is GMP medium available? GMP medium of StemFit For MSC is in preparation. Please contact us directly.

Trouble shooting

    Cells cannot attach the plate after seeding

  • ・Please check if iMatrix-511 is added to the medium or ECM coated plate is used. StemFit For MSC medium does not contain ECM.
  • ・Please check if dissociation reagent was completely eliminated after centrifugation. Remaining dissociation reagent inhibits attachment.  

    Cells cannot detach from the plate during passage

  • ・Please check if cells are not more than 70% confluent. When cells reach to 90-100% confluent, it would be difficult to detach cells.

    Cells do not become single cells after dissociation

  • ・Please check if cells are not more than 70% confluent. When cells reach to 90-100% confluent, it can be difficult to make single cells. Please passage your cells before being 70% confluent.
  • ・Please pipette the cells in the Cell Detachment Solution to fully dissociate cells. Please also refer step. No.5 of cell expansion protocol in the product information.
iPSC/ESC medium Differentiation medium Recombinant Proteins

For more information, please contact us.

stemfit@ajinomoto.com

AJINOMOTO CO., INC. AminoScience Division
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan
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