StemFit™ For MSC
StemFit For Mesenchymal Stem Cell (StemFit For MSC) is a chemically defined medium for mesenchymal stem cell (MSC) culture. This medium does not contain any undefined components such as serum or human platelet lysate, and it enables the maintenance of human bone marrow-derived MSC (BM-MSC), umbilical cord-derived MSC (UC-MSC) and adipose-derived stem cells (ADSC), under chemically defined conditions.
— Materials Provided —
|Store at -20 °C
— Media Preparation —
Use sterile techniques to prepare StemFit For MSC medium.
1) Thaw StemFit medium at room temperature (15-25 °C) or at 2-8°C overnight. Mix thoroughly. If precipitations are observed, keep the bottle at room temperature and dissolve them.
CAUTION: Do not thaw StemFit For MSC at 37 °C, as it accelerates medium degradation
＞ Thawed StemFit For MSC medium may be stored at 2-8 °C for up to a month. Protect from light.
＞ Optionally, the medium can be stored as aliquots at -20°C until the expiration date. Do not re-freeze thawed aliquots.
2) Warm medium to room temperature and use immediately.
Key points for successful culture
Do not thaw the media at 37°C because it accelerates degradation of media.
Add extracellular matrix (ECM) or use ECM coated plate. Please refer to the protocol for details.
Remove dissociation reagent completely after centrifugation.
Avoid over confluent culture because that makes it difficult to detach and collect cells.
— Precaution and Disclaimer —
StemFit For MSC is designed in accordance with the BSE/TSE guideline (EMEA/410/01 Rev.3). Any animal- and human-derived components are properly inactivated and the risk associated with the animal-derived ingredient is negligible.
We define “Chemically Defined Medium” as
1. A medium in which all of the components and concentrations are known.
2. A medium which does not contain serum, lysates, or other ingredients with unknown composition