Feeder free medium for ES/iPS cells StemFit

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StemFit Puroteins Recombinant Proteins Animal-origin free proteins StemFit Puroteins Recombinant Proteins Animal-origin free proteins

Product Information
SCF

SCF
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— Product Size —

Non GMP

10 μg (0.1 mg/ml, 100 μl)
SP-SCF-R-010UG

50 μg (0.1 mg/ml, 500 μl)
SP-SCF-R-050UG

1 mg (0.1 mg/ml, 10 ml)
SP-SCF-R-001MG

Benefits

Significant cost reduction of cell
manufacturing processes

High lot-to-lot consistency

Seamless transition from basic research
to clinical applications

Simplified protocols without
reconstitution

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— Product Details —

NAME Recombinant Human SCF
SOURCE Corynebacterium glutamicum*
FORMULATION Frozen in 20 mM HEPES
Quality Animal origin free
AA Sequence: EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV
QLSDSLTDLL DKFSNISEGL SNYSIIDKLV
NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN
RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVA
Purity: ≥ 95% by SDS-PAGE
Endotoxin <0.1EU/ug
Description: SCF (stem cell factor) is a growth factor secreted from fibroblasts and endothelial cells. SCF binds to a receptor known as c-Kit and is thought to play an important role in the survival, proliferation and differentiation of hematopoietic stem cells. Recombinant Human SCF is an 18.5 kDa polypeptide containing 164 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

*Corynebacterium glutamicum

  • • Discovered in 1957 as a glutamate producer
  • • Fast growing soil bacterium
  • • Gram-positive
  • • Non-sporulating
  • • Non-pathogenic
  • • Non-endotoxic
  • • FDA approved
*Corynebacterium glutamicum

< Features >

  • Secretion System
  • No Endotoxin
  • Less Derivatives (impurities)
  • Experience: Food Amino Acid Production

Ideal platform for low-cost, high-grade proteins production with high lot-to-lot consistency.

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— Product Comparison Table —

AJINOMOTO Company A Company B Company C
Host cell C. glutamicum E. coli CHO Human
Endotoxin <0.1EU/μg
(LAL)
<1EU/μg
(LAL)
<1.0EU/μg
(LAL)
<1EU/μg
(LAL)
Purity 95%
(SDS-PAGE)
≧98%
(SDS-PAGE、HPLC)
>97%
(SDS-PAGE)
>97%
(SDS-PAGE)
Biological
activity
EC50, 1.0-20 ng/mL*1 ED50, ≦2.0 ng/ml*2 ED50, ≦1-5 ng/ml*2 ED50, ≦5 ng/ml*2
Form Frozen
(in 20mM HEPES)
Lyophilized Lyophilized Lyophilized
N-terminal Glu-Gly-Ile Met-Glu-Gly Met-Glu-Gly Met-Glu-Gly
Expiration date 2 years from
manufacturing date
At least 4 years from
manufacturing date
12 months from
date of receipt
  • *1 Phosphorylation assay of c-KIT
  • *2 Cell proliferation assay using TF‑1 human erythroleukemic cells
〈 Comparison of biological activity with other companies’ products 〉

Phosphorylation assay of c-KIT

〈 Comparison of biological activity with other companies’ products 〉

AJINOMOTO’s SCF demonstrates equal to biological
activity compared to tested alternatives!

〈 Stability information after reconstitution 〉
AJINOMOTO Company A Company B
-80°C 2 years
from manufacturing date
1 year from
reconstitution date
3 months from
reconstitution date
4°C At least 1 month 1 month from
reconstitution date
1 month from
reconstitution date
Freeze-thaw cycles Within 3 times
Download

・Product Information
SCF [1mg](PDF [261KB]) SCF [10μg](PDF [266KB]) SCF [50μg](PDF [266KB])

・SDS (PDF [127KB] / En) (PDF [138KB] / JP)

iPSC/ESC medium Differentiation medium MSC medium

For more information, please contact us.

Contact us
AJINOMOTO CO., INC. AminoScience Division
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan

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