Feeder free medium for ES/iPS cells StemFit

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stemfit@ajinomoto.com

stemfit@ajinomoto.com
Technical Support Technical Support

Movies

Establishment of iPS cells by the episomal method
(1) Preparation of plates

Establishment of iPS cells by the episomal method
(2) establishment by the episomal method

Passage method
(1) Preparation of plates and medium

Passage method
(2) Passage

Freezing
(1) Preparation of medium and detachment of cells

Freezing
(2) Cell freezing

Publications

Development for StemFit media

  • Sci.Rep. 2014 Jan 8;4:3594.
    A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.

Maintenance protocol for hPSCs with StemFit Basic02 medium

  • Nat Protoc. 2017 Jan;12(1):195-207.
    Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.
  • Curr Protoc Stem Cell Biol. 2018 e50
    CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.

Maintenance protocol for primate PSCs with StemFit medium

  • Sci Data. 2017 Jun 20;4:170067.
    Single-cell Transcriptome of Early Embryos and Cultured Embryonic Stem Cells of Cynomolgus Monkeys
  • Sci Rep. 2018 Aug 15;8(1):12187
    Derivation of Induced Pluripotent Stem Cells in Japanese Macaque (Macaca Fuscata)

Application for genome-edited clone generation

  • Methods. 2016 May 15;101:43-55.
    Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives.
  • Stem Cell Reports. 2016 Apr 12;6(4):496-510.
    Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells.
  • Hum Mol Genet. 2016 Oct 18.
    Genetic and pharmacological correction of aberrant dopamine synthesis using patient iPSCs with BH4 metabolism disorders.
  • Curr Protoc Stem Cell Biol. 2018 e50
    CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.

Liver & Gut organoids

  • Cell Rep. 2017 Dec 5;21(10):2661-2670.
    Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells.
  • Nature. 2017 Jun 22;546(7659):533-538.
    Multilineage communication regulates human liver bud development from pluripotency.
  • Stem Cell Reports. 2018 Mar 13;10(3):780-793.
    Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of Forming Gut and Liver Organoids.

Kidney organoids

  • Cell Stem Cell. 2017 Dec 7;21(6):730-746.e6.
    Higher-Order Kidney Organogenesis from Pluripotent Stem Cells.
  • Nat Protoc. 2017 Jan;12(1):195-207.
    Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.
  • Methods Mol Biol. 2017;1597:179-193.
    Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells.

Differentiation

  • PLoS One. 2014 Dec 2;9(12):e112291.
    Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media.
  • Stem Cell Res. 2017 Dec;25:98-106.
    A human iPS cell myogenic differentiation system permitting high-throughput drug screening.
  • Stem Cell Reports. 2017 Nov 14;9(5):1406-1414.
    Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes.
  • Stem Cell Reports. 2016 Mar 8;6(3):312-20.
    Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates.
  • Nature. 2017 Aug 30;548(7669):592-596.
    Human iPS cell-derived dopaminergic neurons function in a primate Parkinson's disease model.
  • Mol Brain. 2016 Sep 19;9(1):85.
    Pathological classification of human iPSC-derived neural stem/progenitor cells towards safety assessment of transplantation therapy for CNS diseases.

For further information, please contact here.

stemfit@ajinomoto.com

AJINOMOTO CO., INC. AminoScience Division
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan
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