Feeder free medium for ES/iPS cells StemFit Basic02

Publications

Development for StemFit media

  • Sci.Rep. 2014 Jan 8;4:3594.
    A novel efficient feeder-free culture system for the derivation of human induced pluripotent stem cells.

Maintenance protocol for hPSCs with StemFit Basic02 medium

  • Nat Protoc. 2017 Jan;12(1):195-207.
    Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.
  • Curr Protoc Stem Cell Biol. 2018 e50
    CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.

Application for genome-edited clone generation

  • Methods. 2016 May 15;101:43-55.
    Engineering the AAVS1 locus for consistent and scalable transgene expression in human iPSCs and their differentiated derivatives.
  • Stem Cell Reports. 2016 Apr 12;6(4):496-510.
    Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells.
  • Hum Mol Genet. 2016 Oct 18.
    Genetic and pharmacological correction of aberrant dopamine synthesis using patient iPSCs with BH4 metabolism disorders.
  • Curr Protoc Stem Cell Biol. 2018 e50
    CRISPR/Cas9-based Targeted Genome Editing for the Development of Monogenic Diseases Models with Human Pluripotent Stem Cells.

Liver & Gut organoids

  • Cell Rep. 2017 Dec 5;21(10):2661-2670.
    Massive and Reproducible Production of Liver Buds Entirely from Human Pluripotent Stem Cells.
  • Nature. 2017 Jun 22;546(7659):533-538.
    Multilineage communication regulates human liver bud development from pluripotency.
  • Stem Cell Reports. 2018 Mar 13;10(3):780-793.
    Human iPSC-Derived Posterior Gut Progenitors Are Expandable and Capable of Forming Gut and Liver Organoids.

Kidney organoids

  • Cell Stem Cell. 2017 Dec 7;21(6):730-746.e6.
    Higher-Order Kidney Organogenesis from Pluripotent Stem Cells.
  • Nat Protoc. 2017 Jan;12(1):195-207.
    Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells.
  • Methods Mol Biol. 2017;1597:179-193.
    Generation of a Three-Dimensional Kidney Structure from Pluripotent Stem Cells.

Differentiation

  • PLoS One. 2014 Dec 2;9(12):e112291.
    Derivation of mesenchymal stromal cells from pluripotent stem cells through a neural crest lineage using small molecule compounds with defined media.
  • Stem Cell Res. 2017 Dec;25:98-106.
    A human iPS cell myogenic differentiation system permitting high-throughput drug screening.
  • Stem Cell Reports. 2017 Nov 14;9(5):1406-1414.
    Efficient Large-Scale 2D Culture System for Human Induced Pluripotent Stem Cells and Differentiated Cardiomyocytes.
  • Stem Cell Reports. 2016 Mar 8;6(3):312-20.
    Cardiomyocytes Derived from MHC-Homozygous Induced Pluripotent Stem Cells Exhibit Reduced Allogeneic Immunogenicity in MHC-Matched Non-human Primates.
  • Nature. 2017 Aug 30;548(7669):592-596.
    Human iPS cell-derived dopaminergic neurons function in a primate Parkinson's disease model.
  • Mol Brain. 2016 Sep 19;9(1):85.
    Pathological classification of human iPSC-derived neural stem/progenitor cells towards safety assessment of transplantation therapy for CNS diseases.

Conference presentation

ISSCR2018 at Melbourne(Read more)

ISSCR2017 at Boston

  • June 16, 2017, Ajinomoto Innovation Showcase; symposium title “Genome editing of hPSCs for accurate modeling of human diseases”, Chair: Ryuji Morizane, MD, PhD from Brigham Women’s Hospital, Speaker: Ryuji Morizane, MD, PhD and Curtis Robert Warren, PhD from Harvard University (PDF Download)
  • June 16, 2017, Poster# F-2022; title “CRYOPRESERVING AND THAWING CRYOPRESERVED HUMAN PLURIPOTENT STEM CELLS CULTURED IN STEMFIT”, by Yusuke Natsume et al. from Ajinomoto
  • June 15, 2017, Poster# T-2156; title “SUPERIOR CLONING EFFICIENCY OF HUMAN PLURIPOTENT STEM CELLS CULTURED IN A XENO-FREE AND DEFINED CULTURE MEDIUM, STEMFIT”, by Takuya Matsumoto et al. from Ajinomoto

WSCS2016 at West Palm Beach

  • Japan symposium; title “Development of a novel xeno-free medium, StemFit, for human pluripotent stem cells” by Hiroki Ozawa from Ajinomoto

ISSCR2016 at San Francisco

  • June 24, 2016, Ajinomoto Innovation Showcase; symposium title “The generation and expansion of high -quality human pluripotent stem cells and their derivatives for applications in medical science”, Chair: Peter Andrews, PhD from University of Sheffield, UK, Speaker: Peter Andrews, PhD and Kiichiro Tomoda, PhD from Gladstone Institute (PDF Download)
  • June 23, 2016, Poster# T2085; title “EFFECT OF EXTRACELLULAR MATRICES ON THE COLONY FORMING EFFICIENCY OF HUMAN PLURIPOTENT STEM CELLS CULTURED IN STEMFIT™AK.”, by Jessica Chang et al. from Ajinomoto
  • June 22, 2016, Poster# W2215; title “HUMAN PLURIPOTENT STEM CELLS DISPLAY MECHANISTICALLY INDEPENDENT REQUIREMENTS FOR ARGININE IN SURVIVAL AND MAINTENANCE OF PLURIPOTENCY.”, by Hiroki Ozawa et al. from Ajinomoto

WSCS2015 at Atlanta

  • Japan symposium; title “Development of a novel xeno-free medium, StemFit, for human pluripotent stem cells” by Jessica Chang from Ajinomoto

Technical tips

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FAQ

  • What is the difference against other media? StemFit is a medium for single cell expansion for hPSCs.
  • What are the benefits of single cell culture?
    • Superior and stable growth performance
    • High colony forming efficiency (essential for genome editing)
    • Robust scalable cell expansion yet cost-effective
  • Can I use StemFit for the clump culture? Yes, but recommend to make small clump.
  • What is the difference between StemFit AK02N and Basic02? Basically they are same. AK02N contain bFGF but Basic02 does not.

Link

Establishment of iPS cells by the episomal method
(1) Preparation of plates

Establishment of iPS cells by the episomal method
(2) establishment by the episomal method

Passage method
(1) Preparation of plates and medium

Passage method
(2) Passage

Freezing
(1) Preparation of medium and detachment of cells

Freezing
(2) Cell freezing

For further information, please contact here.

stemfit@ajinomoto.com

AJINOMOTO CO., INC. AminoScience Division
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan