StemFit™ For Differentiation (GMP)
StemFit™ For Differentiation (Diff.) is
defined & animal origin-free (AOF)
supplement for differentiation of human ES and iPS cells. It is for both research and further manufacturing use.
The QMS (Quality Management System) follows the Ajinomoto System of Quality Assurance (ASQUA). Manufacturing and product release testing of this product are managed in accordance with the GMP standards Ajinomoto formulated for culture media for regenerative medicine. The GMP standards are prepared with reference to ICHQ7 and “PMDA guidance for Aseptic Manufacturing Processes of Regenerative Medicine Products.
Click here to learn more about GMP grade.
Product Information
StemFit™ For Differentiation
StemFit™ For Diff. combined with StemFit™ medium for the hPSCs expansion can enable clinical applications of hPSC-derived cells/tissues by providing the AOF culture systems for both hPSCs expansion and differentiation.
StemFit™ For Diff. is provided as a 5×concentrate and is intended to be used with basal medium (DMEM/F12、RPMI1640、DMEM etc.) and a variety of different induction factors or cytokines.
— Material Provided —
| Volume | Storage |
| 100ml | Store below -20 °C |
— Prepare Differentiation Basic Medium —
StemFit™ For Diff. is provided frozen and should be stored below -20 °C until use. Use sterile techniques to prepare differentiation medium as follow.
1) Before use, thaw the frozen StemFit™ For Diff. with occasionally mixing at room temperature (15-25 °C) or overnight at 4°C.
CAUTION: Do not thaw StemFit™ for Diff. at 37 °C, as it accelerates the degradation of medium
2) Add 100 mL of StemFit™ For Diff. (5X) to 400 mL basal medium (DMEM/F12、RPMI1640、DMEM etc.) and mix thoroughly to make differentiation basic medium. If precipitations are observed, keep the bottle at room temperature and dissolve them.
Optional: StemFit™ For Diff. and differentiation basic medium may be aseptically aliquoted and stored below -20 °C. Once thawed, they may be stored at 2-8 °C for up to 2 weeks (Do not re-freeze.). We recommend storing the medium protected from light.
3) To differentiate each lineage, induction factors or cytokines can be added as specified by differentiation protocols.
4) Before use, warm aliquots to room temperature and use immediately.
Features
High consistencyEnables consistent cell differentiation
Free of undefined components (Serum, Human-derived component). Defined composition minimizes lot-to-lot variation and enables consistent cell differentiation.
High SafetyAnimal origin-free composition
Free of animal- and human-derived components.
Animal origin-free composition minimizes risk of immunogenic contamination.
Example of differentiation induction into hematopoietic progenitor cells (HPCs)
– Results of a user-led comparative evaluation of differentiation supplements
Century Therapeutics, Inc. evaluated iPS-to-HPC differentiation using StemFit™ For Differentiation with the aim of improving T-cell differentiation efficiency. Using an 8-day differentiation process, they confirmed a consistent increase in HPC yield as well as enhanced differentiation efficiency toward lymphoid lineages.
Furthermore, following protocol optimization using StemFit™ For Differentiation, a substantial improvement in HPC yield was observed while maintaining cell viability and the proportion of CD34+CD43+ cells. This trend was consistently reproduced across multiple iPSC lines, including PiPSCs and TiPSCs, and among multiple operators with varying levels of experience.
Lineage-specific differentiation
StemFit™ For Differentiation is useful for lineage-specific differentiation in replacement of serum-free supplement.
Spontaneous differentiation
StemFit™ For Differentiation can support spontaneous differentiation of hiPSCs via EB formation.
- Download
-
・Publications Using StemFit™ For Differentiation (PDF [465KB])
・Brochure (PDF [1.6MB] )
・SDS (PDF [56KB] )
・GMP statement (PDF [581KB])
FAQ
- Which basal medium can be used with StemFit™ For Differentiation (StemFit™ For Diff)? StemFit™ For Diff. can be used with any Basal medium (DMEM, RPIM1640, DMEM/F12) which are used for iPS/ES cell differentiation
- What is the recommended final concentration of StemFit™ For Diff.? We usually recommend final concentration of 20%. For example, mix 100 ml of StemFit™ For Diff. with 400 ml of basal medium to prepare differentiation basic medium. Lineage specific cytokines or small molecules should be added to differentiation basic medium.
- Can I use StemFit™ For Diff. for all lineage? We have confirmed that StemFit™ For Diff. can be used for EB formation and directed differentiation for endodermal, mesodermal and ectodermal lineages. Please refer the product information . There are some cases where protocol optimization would be required. Please contact us directly for more information.
For more information, please contact us.
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan
