Feeder free medium for ES/iPS cells StemFit

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StemFit Puroteins Recombinant Proteins Animal-origin free proteins StemFit Puroteins Recombinant Proteins Animal-origin free proteins

Product Information
Activin A

Non GMP GMP compliant
Request Pricing

— Product Size —

Non GMP

10 μg (0.1 mg/ml, 100 μl)
SP-ActA-R-010UG

50 μg (0.1 mg/ml, 100 μl)
SP-ActA-R-050UG

1 mg (0.1 mg/ml, 10 ml)
SP-ActA-R-001MG

GMP compliant

1 mg (0.1 mg/ml, 10 ml)
SP-ActA-G-001MG

Benefits

Significant cost reduction of cell
manufacturing processes

High lot-to-lot consistency

Seamless transition from basic research
to clinical applications

Simplified protocols without
reconstitution

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— Product Details —

NAME Recombinant human Activin A
SOURCE E. coli
FORMULATION Frozen in 50 mM sodium acetate buffer, pH4.6
Quality Animal origin free
AA Sequence: GLECDGKVNI CCKKQFFVSF KDIGWNDWII APSGYHANYC EGECPSHIAG
TSGSSLSFHS TVINHYRMRG HSPFANLKSC CVPTKLRPMS MLYYDDGQNI
IKKDIQNMIV EECGCS
Purity: ≥ 97% by SDS-PAGE
Endotoxin <0.01EU/ug
Description: Activin A is a member of the TGF-beta superfamily of cytokines and is involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis. Human Activin A is a 26.0 kDa disulfide-linked homodimer of two βA chains, each containing 116 amino acid residues. Activin A is mainly used for stem cell cultivation in order to differentiate the stem cell into endoderm or mesoderm.
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— Product Comparison Table —

I. Non GMP product

AJINOMOTO Company A Company B Company C
Host cell E. coli E. coli CHO Human
Endotoxin <0.01EU/μg
(LAL)
<1EU/μg
(LAL)
<0.01EU/μg
(LAL)
<0.01EU/μg
(LAL)
Purity 97%
(SDS-PAGE)
≧97%
(SDS-PAGE、HPLC)
>95%
(SDS-PAGE)
>95%
(SDS-PAGE)
Biological
activity*1
ED50, 50-150% vs.
WHO std*2
(K562)
ED50, ≦2.0 ng/ml
(MPC-11)
ED50, 0.2-1.2 ng/ml
(K562)
ED50, 0.5-5 ng/ml
(MPC-11)
Form Frozen
(0.1 mg/ml)
Lyophilized Lyophilized Lyophilized
N-terminal Gly-Leu-Glu Gly
Expiration date 4 years from
manufacturing date
1 year from
date of receipt
1 year from
date of receipt
  • *1 Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cell.
  • *2 Activin A WHO international std., NIBSC code: 91/626

II. GMP compliant product

AJINOMOTO Company A Company B Company D
Host cell E. coli E. coli CHO E. coli
Endotoxin <0.01EU/μg
(LAL)
≦0.10EU/μg
(LAL)
<0.10EU/μg
(LAL)
<0.05EU/μg
(LAL)
Purity ≧97%
(SDS-PAGE)
≧98%
(SDS-PAGE、HPLC)
>97%
(SDS-PAGE)
≧97%
(SDS-PAGE)
*include ≦5% oligomers
Biological
activity*1
ED50, 50-150% vs.
WHO std*2
(K562)
ED50, 1.0-5.0 ng/ml
(MPC-11)
ED50, 0.2-1.2 ng/ml
(K562)
0.6–2.5x103 IU/mg
(MPC-11)
Mycoplasma Negative
(NAT)
Negative
(QF-PCR)
Negative
(Ribosomal RNA
hybridization assay)
Sterility Conform
(Membrane filter)
No growth
(Direct inoculation)
No growth
(Direct inoculation)
Form Frozen
(0.1 mg/ml)
Lyophilized Lyophilized Lyophilized
N-terminal Gly-Leu-Glu Gly-Leu-Glu Gly-Leu-Glu Met-Gly-Leu-Glu
Expiration date 4 years from
manufacturing date
5 years from
manufacturing date
3 years from
bottling date
Minimum 6 months
from shipping date
  • *1 Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cell.
  • *2 Activin A WHO international std., NIBSC code: 91/626
〈 Comparison of biological activity with other companies’ GMP products 〉

Measured by its ability to induce hemoglobin expression in K562 human chronic myelogenous leukemia cell.
Activin A WHO international std., NIBSC code: 91/626

〈 Comparison of biological activity with other companies’ GMP products 〉

AJINOMOTO’s Activin A demonstrates equal to
superior biological activity compared to tested alternatives!

〈 Comparison of MS spectrum with other companies’ GMP products 〉

AJINOMOTO

AJINOMOTO

Company X

Company X

Company Y

Company Y

AJINOMOTO’s Activin A also contains significantly less impurities!

〈 Stability information after reconstitution 〉
AJINOMOTO Company A Company B
-80°C 4 years
from manufacturing date
At least 7 months 3 months
4°C At least 6 months 1 week 1 month
Freeze-thaw cycles Within 2 times
(>90% activity)

— Clinical Compatibility —

The PMDA* has officially confirmed the eligibility of Ajinomoto’s Activin A for use in clinical cell therapy production (in Japan).
* Pharmaceuticals and Medical Devices Agency

— Clinical compativility —
Download

・Product Information Activin A [1mg GMP compliant](PDF [286KB]) Activin A [1mg](PDF [281KB]) Activin A [10μg](PDF [286KB]) Activin A [50μg](PDF [284KB])

・SDS (PDF [63kB] )

iPSC/ESC medium Differentiation medium MSC medium

For more information, please contact us.

stemfit@ajinomoto.com

AJINOMOTO CO., INC. AminoScience Division
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan
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