Feeder free medium for ES/iPS cells StemFit

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stemfit@ajinomoto.com

stemfit@ajinomoto.com
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iPSC/ESC medium Feeder free medium for ESC/iPSC Cells iPSC/ESC medium Feeder free medium for ESC/iPSC Cells

Product Information
StemFit Basic04 Complete Type

StemFit Basic04 Complete Type

StemFit Basic04 Complete Type (Basic04CT) is an animal-origin free, defined medium for human pluripotent stem cell culture. This medium includes Basic Fibroblast Growth Factor (bFGF) and can be used for maintaining human ES and iPS cells under feeder-free culture conditions.

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— Materials Provided —

Volume Storage
500 ml Store at below -20 °C

— Media Preparation —

StemFit Basic04 Complete Type is provided frozen medium and can be stored at below -20 °C until use. Use sterile techniques when using this medium.

1) Before use, thaw the frozen Basic04CT with occasionally mixing at room temperature (15-25 °C).
CAUTION : Do not thaw, StemFit Basic04 Complete Type at 37 °C, as it accelerates the degradation of the medium.
If precipitations are observed, keep the bottle at room temperature and dissolve them.1

2) Upon thawing, StemFit Basic04 Complete Type may be aseptically aliquoted and stored at below -20 °C. Thawed StemFit Basic04 Complete Type may be stored at 2-8 °C for up to two weeks.2
We recommend storing the medium protected from light.3

3) Before use, warm aliquots at room temperature and use immediately.

Key points for successful culture

not 37°C

Do not thaw, “StemFit Basic04 Complete Type” at 37 °C, as it accelerates the degradation of medium ingredients.

2weeks

Thawed StemFit Basic04 Complete Type medium may be stored at 2-8 °C for up to two weeks.

not Light

We recommend storing the medium protected from light

— Precaution and Disclaimer —

StemFit Basic04 Complete Type is for research use only and is not intended for diagnostic or therapeutic use.

Features

Animal-Origin Free

Minimize risk of virus contamination and lot-to-lot variation with animal-origin free formula.

Animal-Origin Free

One Bottle Composition

Achieve sterilization by simplifying the procedure with ready to use packaging.

One Bottle Composition

Weekend-Free Feeding

Skip feeding on weekends for flexible scheduling.

Weekend-Free Feeding

Single-Cell Expansion

Experience superior expansion with single-cell passaging and consistent performance with various matrices.

Single-Cell Expansion
Download

・Product Information (PDF [227KB])

・SDS (PDF [322KB] )

・Brochure (PDF [1.3MB] )

・Instruction Manual
StemFit medium (PDF [542KB] )
StemFit medium with iMatrix-511 (PDF [698KB] )

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StemFit Basic04 Complete Type
FAQ

  • Which Extracellular Matrix (ECM) is recommended? Any commercially available ECMs for hPSCs will work (e.g. iMatrix-511, Geltrex, VTN-N, LN521, Matrigel and Synthemax) .
  • Can I use Basic04 Complete Type (Basic04CT) for clump passaging? We recommend single-cell passaging.  If you do passage cells in clumps, we recommend to make small clumps and seed at low seeding density.
  • How should I switch medium to Basic04? We recommend to switch to Basic04CT 2 days before passaging.
    Please refer to Technical Tips for more details
  • Can I use Basic04CT for non-human PSCs? StemFit medium has been developed for human PSCs, however there are reports in which primate PSCs have been cultured in StemFit medium
  • Can I use Basic04CT for Mesenchymal stem cell or hematopoietic stem cells? No. StemFit For MSC medium is available to expand MSC.
  • Is GMP medium available? GMP medium of Basic04 is in preparation. Please contact us directly.
  • What’s the difference between Basic04 and Basic04CT? Basic04 does not contain bFGF, therefore bFGF can be added and optimized for a specific purpose.  Basic04CT does contain bFGF and can be used for PSC expansion without additional supplement.  
  • What’s the difference between Basic04 and Basic03 The performance of Basic04 and Basic03 is comparable. In contrast to Basic03, all of the media components are packaged in one bottle.
Differentiation medium MSC medium Recombinant Proteins

For more information, please contact us.

stemfit@ajinomoto.com

AJINOMOTO CO., INC. AminoScience Division
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan
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