StemFit Basic04 Complete Type
StemFit Basic04 Complete Type (Basic04CT) is an animal-origin free, defined medium for human pluripotent stem cell culture. This medium includes Basic Fibroblast Growth Factor (bFGF) and can be used for maintaining human ES and iPS cells under feeder-free culture conditions.
— Materials Provided —
||Store at below -20 °C
— Media Preparation —
StemFit Basic04 Complete Type is provided frozen medium and can be stored at below -20 °C until use. Use sterile techniques when using this medium.
1) Before use, thaw the frozen Basic04CT with occasionally mixing at room temperature (15-25 °C).
CAUTION : Do not thaw, StemFit Basic04 Complete Type at 37 °C, as it accelerates the degradation of the medium.
If precipitations are observed, keep the bottle at room temperature and dissolve them.1
2) Upon thawing, StemFit Basic04 Complete Type may be aseptically aliquoted and stored at below -20 °C. Thawed StemFit Basic04 Complete Type may be stored at 2-8 °C for up to two weeks.2
We recommend storing the medium protected from light.3
3) Before use, warm aliquots at room temperature and use immediately.
Key points for successful culture
Do not thaw, “StemFit Basic04 Complete Type” at 37 °C, as it accelerates the degradation of medium ingredients.
Thawed StemFit Basic04 Complete Type medium may be stored at 2-8 °C for up to two weeks.
We recommend storing the medium protected from light
— Precaution and Disclaimer —
StemFit Basic04 Complete Type is for research use only and is not intended for diagnostic or therapeutic use.
Minimize risk of virus contamination and lot-to-lot variation with animal-origin free formula.
One Bottle Composition
Achieve sterilization by simplifying the procedure with ready to use packaging.
Skip feeding on weekends for flexible scheduling.
Experience superior expansion with single-cell passaging and consistent performance with various matrices.