Feeder free medium for ES/iPS cells StemFit Basic02

Product Information : StemFit Basic02

StemFit Basic02 is a xeno-free, defined medium for human pluripotent stem cell culture. This medium is capable of reprogramming, maintaining and differentiating human ES and iPS cells under feeder-free culture conditions.

Materials Provided1

Component Volume Storage
Liquid A 400ml Store at below -20 °C
Liquid B 100ml Store at below -20 °C

Media Preparation

StemFit Basic02 is provided frozen as a 2 component set containing “Liquid A” and “Liquid B” and can be stored at below -20 °C until use. Use sterile techniques to prepare StemFit Basic02 medium.

1) Before use, thaw the frozen “Liquid A” and “Liquid B” with occasionally mixing at room temperature (15-25 °C). 2

2) Aseptically mix medium components by adding the full volume of “Liquid B” to “Liquid A”. Mix thoroughly. If prepared aseptically, StemFit Basic02 is ready for use.

3) Upon thawing, StemFit Basic02 medium may be aseptically aliquoted and stored at below -20 °C.34

4) Before use, warm aliquots to room temperature and use immediately.

bFGF not contained

This product does not contain basic fibroblast growth factor (bFGF)

not 37°C

Do not thaw, “Liquid B” at 37 °C, as it accelerates the degradation of medium ingredients.

2weeks

Thawed StemFit Basic02 medium may be stored at 2-8 °C for up to two weeks.

not Light

We recommend storing the medium protected from light

Precaution and Disclaimer

StemFit Basic02 is for research use only and is not for diagnostic use, therapeutic use (including clinical research), or as a food. This product contains material derived from human plasma. Therefore, this product should be treated as a potentially infectious agent, and in accordance with universal handling procedures.

For Research Use Only. Not Intended For Human or Animal Diagnostic or Therapeutic Uses
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basic03 distributors technical support

For further information, please contact here.

stemfit@ajinomoto.com

AJINOMOTO CO., INC. AminoScience Division
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan