Feeder free medium for ES/iPS cells StemFit

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stemfit@ajinomoto.com

stemfit@ajinomoto.com
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iPSC/ESC medium Feeder free medium for ESC/iPSC Cells iPSC/ESC medium Feeder free medium for ESC/iPSC Cells

Product Information
StemFit Basic03 GMPCat No:34770

StemFit Basic03 GMP

StemFit Basic03 GMP is an animal-origin free, defined medium for human pluripotent stem cell culture. This medium is capable of reprogramming, maintaining and differentiating human ES and iPS cells under feeder-free culture conditions.This product is manufactured under GMP guidelines by a certified ISO 13485 facility.

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— Materials Provided —1

Component Volume Storage
Liquid A 400ml Store at below -20 °C
Liquid B 100ml Store at below -20 °C

Note: This product does not contain basic fibroblast growth factor (bFGF)

— Media Preparation —

StemFit Basic03 GMP is provided frozen as a 2 component set containing “Liquid A” and “Liquid B” and can be stored at below -20 °C until use. Use sterile techniques to prepare StemFit Basic03 GMP medium.

1) Before use, thaw the frozen “Liquid A” and “Liquid B” with occasionally mixing at room temperature (15-25 °C).
CAUTION : Do not thaw, “Liquid B” at 37 °C, as it accelerates the degradation of medium 2

2) Upon thawing, StemFit Basic03 GMP medium may be aseptically aliquoted and stored at below -20 °C. Thawed StemFit Basic03 GMP medium may be stored at 2-8 °C for up to two weeks. 3
Optional: We recommend storing the medium protected from light4

3) Before use, warm aliquots to room temperature and use immediately.

Key points for successful culture

bFGF not contained

This product does not contain basic fibroblast growth factor (bFGF)

not 37°C

Do not thaw, “Liquid B” at 37 °C, as it accelerates the degradation of medium ingredients.

2weeks

Thawed StemFit Basic03 GMP medium may be stored at 2-8 °C for up to two weeks.

not Light

We recommend storing the medium protected from light

— Precaution and Disclaimer —

StemFit Basic03 GMP is for research and further manufacturing use only. It is not for diagnostic use, therapeutic use, or as a food.

For Research and Further Manufacturing Use Only. Not Intended For Human or Animal Diagnostic or Therapeutic Uses
Download

・Product Information (PDF [230KB])

・SDS (PDF [341KB] )

・Instruction Manual
StemFit medium (PDF [542KB] )
StemFit medium with iMatrix-511 (PDF [698KB] )

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StemFit Basic03 GMP
FAQ

  • Can I switch medium from Basic04 to Basic03 GMP? We confirmed that switching from Basic04 to Basic03 or Basic03 GMP is very smooth. We recommend to switch to Basic03 GMP 2 days before passaging.
  • Which bFGF can be used for expansion? Ajinomoto provides high quality, animal-origin free bFGF, however commercially available bFGF have been confirmed to work.
  • Which Extracellular Matrix (ECM) is recommended? Any commercially available ECMs for hPSCs will work (e.g. iMatrix-511, Geltrex, VTN-N, LN521, Matrigel and Synthemax) .
  • Can I use Basci03 GMP for clump passaging? We recommend single-cell passaging.  If you do passage cells in clumps, we recommend to make small clumps and seed at low seeding density.
  • How should I switch medium to Basic03 GMP? We recommend to switch to Basic03 GMP 2 days before passaging.
    Please refer to Technical Tips for more details
  • Can I use Basic03 GMP for non-human PSCs? StemFit medium has been developed for human PSCs, however there are reports in which primate PSCs have been cultured in StemFit medium
  • Can I use Basic03 GMP for Mesenchymal stem cell or hematopoietic stem cells? No. StemFit For MSC medium is available to expand MSC.
  • What’s the difference between Basic03 GMP and Basic03? Basic03 GMP corresponds to Basic03, but is manufactured under GMP guidelines by a Certified ISO 13485 facility
Differentiation medium MSC medium Recombinant Proteins

For more information, please contact us.

stemfit@ajinomoto.com

AJINOMOTO CO., INC. AminoScience Division
15-1, Kyobashi 1-Chome, Chuo-Ku, Tokyo 104-8315, Japan
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